T3526

Immunoblotting
Chicken gizzard extract was separated on SDS-PAGE and probed with AB TO TUBULIN (RB) (Prepack) (Cat. No. T3526). The antibody was developed with HRP APA Rabbit IgG (GT) (Cat. No. A0545) and an AEC substrate.
Antibody concentration 1/50.

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T3526

Sigma

 

Anti-Tubulin antibody produced in rabbit

whole antiserum

MDL number:MFCD00145921

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Description

Immunogenmicrotubule proteins from brains of 13 day old chicken embryos
SpecificityThe product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Properties

antibody formwhole antiserum
clone(polyclonal)
contains15 mM sodium azide
species reactivitymouse, chicken
application(s)immunoblotting: 1:50 using chicken gizzard extract
 indirect immunofluorescence: 1:80 using cultured fibroblasts
Gene Informationmouse ... Tuba1b(22143), Tubb2a(22151), Tubb2b(73710), Tubb2c(227613)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

References

referenceRisco, C., et al., Endoplasmic Reticulum-Golgi Intermediate Compartment Membranes and Vimentin Filaments Participate in Vaccinia Virus Assembly J. Virol. 76, 1839-1855, (2002) Abstract
 Risco, C., et al., Polymorphism and Structural Maturation of Bunyamwera Virus in Golgi and Post-Golgi Compartments J. Virol. 77, 1368-1381, (2003) Abstract
 Yochum, G.S., et al., Serial analysis of chromatin occupancy identifies β-catenin target genes in colorectal carcinoma cells Proc. Natl. Acad. Sci. U. S. A. 104, 3324-3329, (2007) Abstract